84-5557-63　［取扱停止］PKA II alpha reg 30ul　541054

特徴

• cAMP is a signaling molecule important for a variety of cellular functions.
• cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins.
• The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits.
• cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
• Four different regulatory subunits and three catalytic subunits have been identified in humans.
• The protein encoded by PRKAR2A (protein kinase cAMP-dependent type II regulatory subunit alpha) is one of the regulatory subunits.
• This subunit can be phosphorylated by the activated catalytic subunit.
• It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase.
• This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.