Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position.
The enzyme comprises a 110kD catalytic subunit and a regulatory subunit of either 85kD, 55kD, or 50kD.
PIK3R1 encodes the 85kD regulatory subunit.
Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in PIK3R1 has been associated with insulin resistance.
Alternative splicing of PIK3R1 results in four transcript variants encoding different isoforms.
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human PI 3-kinase p85alpha. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the Internal region of human PI 3-kinase p85alpha.
