Protein phosphatase involved in regulation of Akt and PKC signaling.
Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on ‘Ser-473' of AKT2 and AKT3, ‘Ser-660' of PRKCB and ‘Ser-657' of PRKCA (PubMed: 15808505, PubMed: 17386267, PubMed: 18162466).
Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons (By similarity).
Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes.
Dephosphorylation of ‘Ser-473' of Akt triggers apoptosis and suppression of tumor growth.
Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation (PubMed: 18162466).
Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed: 20513427).
Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed: 21986499).
Inhibits cancer cell proliferation and may act as a tumor suppressor (PubMed: 19079341).
Dephosphorylates RAF1 inhibiting its kinase activity (PubMed: 24530606).
May act as a negative regulator of K-Ras signaling in membrane rafts (By similarity).
Involved in the hippocampus-dependent long-term memory formation (By similarity).
Involved in circadian control by regulating the consolidation of circadian periodicity after resetting (By similarity).
Involved in development and function of regulatory T-cells (By similarity).
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human PHLP1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human PHLP1.
