84-5554-36　［取扱停止］PEBP2 beta 30ul　540882

特徴

• Core-binding factor beta subunit encoded by CBFB is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.
• G.
• RUNX1) and osteogenesis (e.
• G.
• RUNX2).
• The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters.
• Alternative splicing generates two mRNA variants, each encoding a distinct C-terminal.
• In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N-terminal of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11.
• This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype.
• Two transcript variants encoding different isoforms have been found for CBFB.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.