84-5554-05　［取扱停止］PDP1 100ul　540866

特徴

• Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex.
• Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex.
• Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases.
• Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits.
• Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver.
• The catalytic subunit, encoded by PDP1, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily.
• Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix.
• Mutation in PDP1 causes pyruvate dehydrogenase phosphatase deficiency.
• Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.