84-5552-65　［取扱停止］PCBP2 30ul　540782

特徴

• PCBP2 (Poly(RC) binding protein 2) is a protein coding gene.
• Among its related pathways are translational control and mRNA splicing - major pathway.
• The protein encoded by PCBP2 appears to be multifunctional.
• Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins.
• The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding.
• Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure.
• The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
• This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2.
• PCBP2 and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes.
• PCBP2 also has two processed pseudogenes (PCBP2P1 and PCBP2P2).
• Multiple transcript variants encoding different isoforms have been found for PCBP2.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.