84-5551-92　［取扱停止］Pax-5 30ul　540746

特徴

• PAX5 encodes a member of the paired box (PAX) family of transcription factors.
• The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box.
• Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation.
• This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation.
• Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis.
• This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas.
• This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas.
• Alternative splicing results in multiple transcript variants encoding different isoforms.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.