The loss of a tumor suppressor is most often through large deleterious events, such as frameshift mutations, or premature stop codons.
In TP53 however, many of the observed mutations in cancer are found to be single nucleotide missense variants.
These variants are broadly distributed throughout the gene, but with the majority localizing in the DNA binding domain.
There is no single hotspot in the DNA binding domain, but a majority of mutations occur in positions aa175, 245, 248, 273, and 282 (NM_000546) (Olivier et al.
2010).
While a large proportion of cancer genomics research is focused on somatic variants, TP53 is also of note in the germline.
Germline TP53 mutations are the hallmark of Li-Fraumeni syndrome, and many (both germline and somatic) variants have been found to have a prognostic impact on patient outcomes.
The significance of many polymorphisms for susceptibility and prognosis of disease is still very much up for debate.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human p53 (Acetyl-K305), while it doesn't react with total.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aaK305 from human p53 (Acetyl-K305).
