The protein encoded by NCF1 (neutrophil cytosolic factor 1) is a 47kD cytosolic subunit of neutrophil NADPH oxidase.
This oxidase is a multicomponent enzyme that is activated to produce superoxide anion.
Mutations in NCF1 have been associated with chronic granulomatous disease.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human p47-phox (phospho Ser304). Species Crossreactivity: monkey
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to human p47-phox around the phosphorylation site of S304.
