The protein encoded by NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense.
This protein is preferentially expressed in cells of myeloid lineage.
It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation.
This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system.
The PX domain of this protein can bind phospholipid products of the PI (3) kinase, which suggests its role in PI (3) kinase-mediated signaling events.
The phosphorylation of neutrophil cytosolic factor 4 was found to negatively regulate the enzyme activity.
Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Applications:Suitable for use in Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human p40-phox (phospho Thr154). Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human p40-phox around the phosphorylation site of T154.
