84-5547-63　［取扱停止］p38, phosphorylated (Tyr323) 30ul　540523

特徴

• Mitogen-activated protein kinase 14 encoded by MAPK14 is a member of the MAP kinase family.
• MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development.
• Mitogen-activated protein kinase 14 is activated by various environmental stresses and proinflammatory cytokines.
• The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with mitogen-activated protein kinase 14.The substrates of mitogen-activated protein kinase 14 include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of mitogen-activated protein kinase 14 in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.
• Four alternatively spliced transcript variants of MAPK14 encoding distinct isoforms have been reported.
• Applications:Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.