Secreted phosphoprotein 1 encoded by SPP1 is involved in the attachment of osteoclasts to the mineralized bone matrix.
Secreted phosphoprotein 1 is secreted and binds hydroxyapatite with high affinity.
The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to secreted phosphoprotein 1.
Secreted phosphoprotein 1 is also a cytokine that upregulates expression of interferon-gamma and interleukin-12.
Several transcript variants encoding different isoforms have been found for SPP1.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human OPN. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa230-310 of human OPN at C-terminal.
