POU5F1 encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency.
Aberrant expression of POU5F1 in adult tissues is associated with tumorigenesis.
POU5F1 can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation.
Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms.
One of the AUG start codons is polymorphic in human populations.
Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Oct-3/4. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa160-240 of human Oct-3/4.
