LEP encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis.
Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure.
This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing.
Mutations inLEP and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients.
A mutation in LEP has also been linked to type 2 diabetes mellitus development.
LEP (leptin) is a protein coding gene.
Diseases associated with LEP include obesity, morbid, due To leptin deficiency and obesity due To congenital leptin deficiency.
Among its related pathways are common cytokine receptor gamma-chain family signaling pathways and transcription factor regulation in adipogenesis.
Applications:Suitable for use in Immunohistochemistry, ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: paraffin 1:50-1:200ELISA: 1:10,000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Ob . Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa10-50 from human Ob.
