The nuclear pore complex (NPC) mediates bidirectional macromolecular traffic between the nucleus and cytoplasm in eukaryotic cells and is comprised of more than 100 different subunits.
Many of the subunits belong to a family called nucleoporins (Nups), which are characterized by the presence of O-linked-N-acetylglucosamine moieties and a distinctive pentapeptide repeat (XFXFG).
Nup93 (nucleoporin 93) is the most abundant nucleoporin found per NPC, contributing over 10% of the mass.
It localizes to the nuclear side of the NPC, predominantly in the basket terminal ring area, and exists in a complex with Nup188, Nup53 and Nup205.
This complex is crucial for NPC stability and proper assembly.
Nup93 interacts directly with the Nup62 complex located at the center of the NPC and thus tethers the two subcomplexes.
Nup93 is composed of a coiled-coil domain at its N-terminal and a C-terminal helical domain.
Its proper function is essential for cell viability and normal NPC function.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Nup93. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa190-270 from the Internal region of human Nup93.
