NIFK (nucleolar protein interacting with the FHA domain of MKI67) encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen.
The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression.
Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.
Applications:Suitable for use in Immunohistochemistry, Immunofluorescence, ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of human NIFK (phospho Thr234). Species Crossreactivity: Mouse
Isotype:IgG
Calc Applications Abbrev:E IF IHC
Calc Crossreactivity:Hu Mo
Immunogen:Synthetic peptide corresponding to human NIFK around the phosphorylation site of T234.