NF-kappa-B is a ubiquitous transcription factor involved in several biological processes.
It is held in the cytoplasm in an inactive state by specific inhibitors.
Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes.
NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB.
The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA.
Four transcript variants encoding different isoforms have been found for this gene.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunoprecipitation 2-5 ug/mg lysateELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human NFκB-p65 (phospho Thr435). Species Crossreactivity: mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to human NFkappaB-p65 around the phosphorylation site of T435.
