NFKB2 encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB).
The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function.
The protein encoded by NFKB2 can function as both a transcriptional activator or repressor depending on its dimerization partner.
The p100 full-length protein is co-translationally processed into a p52 active form.
Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins.
There is a pseudogene for this gene on chromosome 18.
Alternative splicing results in multiple transcript variants.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunoprecipitation (lysate): 2-5ug/mgELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human NFkappaB-p100 (phospho Ser869). Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human NFkappaB-p100 around the phosphorylation site of S869.
