The erythroid-specific protein encoded by NFE4, and the ubiquitous transcription factor CP2, form the stage selector protein(SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells.
Alternate use of an in-frame upstream non-AUG(CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms.
While the long isoform (22kD) acts as an activator, the short isoform (14kD) has been shown to repress gamma-globin gene expression.
NFE4 is located in an intron of the FBXL13 gene on the opposite strand.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NF-E4.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the human NF-E4 around the acetylation site of K43.
