84-5529-39　［取扱停止］NF kappa B-p105, phosphorylated (Ser927) 30ul　539607

特徴

• NFKB1 encodes a 105kD protein (nuclear factor kappa B subunit 1) which can undergo cotranslational processing by the 26S proteasome to produce a 50kD protein.
• The 105kD protein is a Rel protein-specific transcription inhibitor and the 50kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex.
• NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products.
• Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions.
• Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth.
• Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.