NCALD (neurocalcin delta) encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins.
The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops.
The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network.
The protein is thought to be a regulator of G protein-coupled receptor signal transduction.
Several alternatively spliced variants of NCALD have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.
Applications:Suitable for use in Immunohistochemistry, ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Neurocalcin delta. Species Crossreactivity: Mouse, Rat
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa370-450 from the Internal region of human Neurocalcin delta.
