84-5525-87　［取扱停止］NARF 30ul　539432

特徴

• Several proteins have been found to be prenylated and methylated at their C-terminal ends.
• Prenylation was initially believed to be important only for membrane attachment.
• However, another role for prenylation appears to be its importance in protein-protein interactions.
• The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins.
• Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a C-terminal CaaX motif.
• This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A.
• The nuclear prelamin A recognition factor encoded by NARF binds to the prenylated prelamin A C-terminal tail domain.
• It may be a component of a prelamin A endoprotease complex.
• The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina.
• It shares limited sequence similarity with iron-only bacterial hydrogenases.
• Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.