Myosin heavy chain 11 encoded by MYH11 is a smooth muscle myosin belonging to the myosin heavy chain family.
The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits.
It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP.
The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter.
The pericentric inversion of chromosome 16 produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N-terminal of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain.
This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype.
Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation.
Alternatively spliced transcript variants encoding different isoforms have been identified.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human MYH11.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human MYH11 protein.
