MUC1 encodes a membrane-bound protein that is a member of the mucin family.
Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces.
These proteins also play a role in intracellular signaling.
Mucin-1 is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas.
Mucin-1 is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex.
The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling.
Overexpression, aberrant intracellular localization, and changes in glycosylation of Mucin-1 have been associated with carcinomas.
MUC1 is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain.
Alternate splicing results in multiple transcript variants.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Mucin 1.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human Mucin 1 around the non-phosphorylation site of Y1229.
