84-5521-63　［取扱停止］MRP-S18C 100ul　539194

特徴

• Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion.
• Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit.
• They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed.
• Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA.
• Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology.
• MRPS18C encodes a 28S subunit protein that belongs to the ribosomal protein S18P family.
• The encoded protein mitochondrial ribosomal protein S18C is one of three that has significant sequence similarity to bacterial S18 proteins.
• The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins.
• Pseudogenes corresponding to this gene are found on chromosomes 8p, 12p, 15q, and 22q.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.