MMP1 encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs).
Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis.
The encoded preproprotein is proteolytically processed to generate the mature protease.
This secreted protease (matrix metallopeptidase 1) breaks down the interstitial collagens, including types I, II, and III.
MMP1 is part of a cluster of MMP genes on chromosome 11.
Mutations in MMP1 are associated with chronic obstructive pulmonary disease (COPD).
Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of fragments of activated human MMP-1 22k protein resulting from cleavage adjacent to F100.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa50-130 of the Internal region of human MMP-1 22k.
