84-5518-59　［取扱停止］MLL 100ul　539039

特徴

• Histone methyltransferase that plays an essential role in early development and hematopoiesis.
• Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of Lys-4 of histone H3 (H3K4me) complex and acetylation of Lys-16 of histone H4 (H4K16ac).
• In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation.
• Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity.
• Has no activity toward histone H3 phosphorylated on Thr-3, less activity toward H3 dimethylated on Arg-8 or Lys-9, while it has higher activity toward H3 acetylated on 'Lys-9'.
• Required for transcriptional activation of HOXA9.
• Promotes PPP1R15A-induced apoptosis.
• Plays a critical role in the control of circadian gene expression and is essential for the transcriptional activation mediated by the CLOCK-ARNTL/BMAL1 heterodimer.
• Establishes a permissive chromatin state for circadian transcription by mediating a rhythmic methylation of 'Lys-4' of histone H3 (H3K4me) and this histone modification directs the circadian acetylation at H3K9 and H3K14 allowing the recruitment of CLOCK-ARNTL/BMAL1 to chromatin (By similarity).
• Applications:Suitable for use in Immunohistochemistry (paraffin), ELISA.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (paraffin): 1:50-1:200ELISA: 1:10,000-1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.