84-5518-21　［取扱停止］MKP-4 100ul　539017

特徴

• The dual specificity phosphatase 9 encoded by DUSP9 is a member of the dual specificity protein phosphatase subfamily.
• These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues.
• They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation.
• Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli.
• This gene product shows selectivity for members of the ERK family of MAP kinases and is localized to the cytoplasm and nucleus.
• Aberrant expression of this gene is associated with type 2 diabetes and cancer progression in several cell types.
• Alternate splicing results in multiple transcript variants.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.