84-5517-71　［取扱停止］MIP-1b 30ul　538993

特徴

• CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta), is a 12kD beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells.
• CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium.
• A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment.
• CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha.
• The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV.
• Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2.
• In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a co-receptor.
• Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5.
• Mature mouse CCL4 shares 77% and 86% amino acid sequence identity with human and rat CCL4, respectively.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.