84-5517-53　［取扱停止］Midline-1 30ul　538984

特徴

• Midline-1 encoded by this MID1 is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins.
• The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
• This protein forms homodimers which associate with microtubules in the cytoplasm.
• Midline-1 is likely involved in the formation of multiprotein structures acting as anchor points to microtubules.
• Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum.
• This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse.
• Multiple different transcript variants are generated by alternate splicing; however, the full-length nature of some of the variants has not been determined.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.