MAP2K4 (mitogen-activated protein kinase kinase 4) encodes a member of the mitogen-activated protein kinase (MAPK) family.
Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development.
They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs.
This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues.
A similar protein in mouse has been reported to play a role in liver organogenesis.
A pseudogene of MAP2K4 is located on the long arm of chromosome X.
Alternative splicing results in multiple transcript variants.
Applications:Suitable for use in Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human MEK-4 (phospho Ser257). Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human MEK-4 around the phosphorylation site of S257.
