Mitogen-activated protein kinase 3 encoded by MAP2K3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family.
Mitogen-activated protein kinase 3 is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade.
It phosphorylates and thus activates MAPK14/p38-MAPK.
Mitogen-activated protein kinase 3 can be activated by insulin, and is necessary for the expression of glucose transporter.
Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells.
The inhibition of Mitogen-activated protein kinase 3 is involved in the pathogenesis of Yersina pseudotuberculosis.
Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for MAP2K3.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immuoprecipitation (lysate): 2-5ug/mgELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human MEK-2 protein only when phosphorylated at T394. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human MEK-2 around the phosphorylation site of T394.
