The protein encoded by MAP2K2 (mitogen-activated protein kinase kinase 2) is a dual specificity protein kinase that belongs to the MAP kinase kinase family.
This kinase is known to play a critical role in mitogen growth factor signal transduction.
It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3.
The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases.
Mutations in MAP2K2 cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome.
The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax.
A pseudogene, which is located on chromosome 7, has been identified for MAP2K2.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunoprecipitation (lysate): 2-5ug/mgELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human MEK-2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human MEK-2 around the non-phosphorylation site of T394.
