MDM2 encodes a nuclear-localized E3 ubiquitin ligase.
The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation.
MDM2 is itself transcriptionally-regulated by p53.
Overexpression or amplification of this locus is detected in a variety of different cancers.
There is a pseudogene for this gene on chromosome 2.
Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human MDM2 (phospho Ser166). Species Crossreactivity: mouse and monkey
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mk Mo
Immunogen：Synthetic peptide corresponding to human MDM2 around the phosphorylation site of S166.
