Maltose binding protein (MBP) is the 370aa product of the E.coli mal E gene.
MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein.
The MBP-Tag also promotes proper folding of the attached protein.
Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin.
Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site.
Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes MBP and MBP-Tag fusion proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Immunogen：Recombinant protein corresponding to MBP-Tag.
