DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development.
MBD3 belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD).
Methyl-CpG-binding domain protein 3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities.
Unlike the other family members, the encoded protein is not capable of binding to methylated DNA.
The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex.
Alternative splicing results in multiple transcript variants of this gene.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human MBD3. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa190-270 of human MBD3 at C-terminal.
