MASP1 encodes a serine protease that functions as a component of the lectin pathway of complement activation.
The complement pathway plays an essential role in the innate and adaptive immune response.
The encoded protein (mannan binding lectin serine peptidase 1) is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins.
Mannan binding lectin serine peptidase 1 is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2.
Mannan binding lectin serine peptidase 1 is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation.
A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway.
Alternate splicing results in multiple transcript variants.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Cleaved-MASP-1 HC (R448).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa370-450 of the Internal region of human MASP-1 HC.
