Microtubules, the primary component of the cytoskeletal network, interact with proteins called microtubule-associated proteins (MAPs).
The microtubule-associated proteins can be divided into two groups, structural and dynamic.
The MAP proteins function to stimulate tubulin assembly, enhance microtubule stability, influence the spatial distribution of microtubules within cells and utilize microtubule polarity to translocate cellular components.
MAP-9 (microtubule-associated protein 9), also known as ASAP, is a 647 amino acid cytoplasmic protein that is constitutively expressed during the cell cycle.
MAP-9 localizes to microtubules in interphase, associates with the mitotic spindle during mitosis and localizes to the central body during cytokinesis.
Involved in organization of the bipolar mitotic spindle, MAP-9 is required for bipolar spindle assembly, mitosis progression and cytokinesis.
MAP-9 may be involved in stabilizing interphase microtubules.
Two isoforms of MAP-9 are produced due to alternative splicing events.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human MAP-9.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa90-170 from the Internal region of human MAP-9.
