The protein encoded by MAP4 is a major non-neuronal microtubule-associated protein.
This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU).
This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion.
Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules.
The phosphorylation of this protein affects microtubule properties and cell cycle progression.
Multiple transcript variants encoding different isoforms have been found for this gene.
Applications:Suitable for use in Immunohistochemistry, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human MAP-4 (phospho Ser696). Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human MAP-4 around the phosphorylation site of S696.
