The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs).
LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2).
NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 612386).
NFE2 DNA-binding activity consists of a heterodimer containing an 18kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490).
Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).
Maf homodimers suppress transcription at NFE2 sites.
MAFK (MAF BZIP Transcription Factor K) is a Protein Coding gene.
Diseases associated with MAFK include fibrosarcoma.
Among its related pathways are Response to elevated platelet cytosolic Ca2+ and factors involved in megakaryocyte development and platelet production.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human MAFK. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human MAFK protein.
