MAP3K12 (mitogen-activated protein kinase kinase kinase 12) encodes a member of the serine/threonine protein kinase family.
This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells.
The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin.
This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation.
Alternatively spliced transcript variants encoding different proteins have been described.
MAP3K12 is a protein coding gene.
Among its related pathways are IL-2 pathway and NFAT and cardiac hypertrophy.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human M3K12. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa730-810 from human M3K12.
