The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis.
A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases.
LRRK1 (leucine-rich repeat serine/threonine-protein kinase 1), also known as RIPK6, Roco1 or KIAA1790, is a 2038aa cytoplasmic protein that contains one protein kinase domain, four ANK repeats and 11 leucine-rich repeats and belongs to the Ser/Thr protein kinase family.
Using magnesium as a cofactor, LRRK1 catalyzes the ATP-dependent phosphorylation of target proteins.
The gene encoding LRRK1, which is expressed as four alternatively spliced isoforms, is thought to be involved in the pathogenesis of Parkinson's disease.
Applications:Suitable for use in Immunohistochemistry, Immunofluorescence, ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human LRRK1.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa330-410 from the Internal region of human LRRK1.
