NLRP3, also known as NALP3, CIAS1, PYPAF or Cryopyrin, is a cytosolic 120kD member of the NLRP family of proteins expressed in leukocytes, especially neutrophils.
As a component of the inflammasome, NLRP3 activates caspases 1 and 5.
Defects in NLRP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes.
Mouse NLRP3 contains an N-terminal Pyrin domain (aa1-91) followed by a Nacht region (aa216-532), and seven LRRs (aa739-988).
Within the sequence used as an immunogen, mouse NLRP3 shares 78% and 93% aa identity with human and rat NLRP3, respectively.
Alternate splicing of mouse NLRP3 generates additional isoforms that lack either LRR2 and 3, LRR 6 and 7, or LRR4-9.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human LRP3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa30-110 of human LRP3 at N-terminal.
