TUSC5 (tumor suppressor candidate 5), also known as protein located at seventeen-p-thirteen point three 1, LOST1 or IFITMD3 (interferon-induced transmembrane domain-containing protein D3), is a 177aa multi-pass membrane protein that belongs to the CD225 family.
Thought to play a role in fat metabolism, TUSC5 is highly expressed in mammary gland, heart, smooth muscle, skeletal muscle and stomach, with lower levels found in lung and brain.
The gene encoding TUSC5 maps to human chromosome 17, which comprises over 2.
% of the human genome and encodes over 1200 genes.
Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1.
Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome.
Like p53, BRCA1 is directly involved in DNA repair, and is liked to predisposition of cancers of the ovary, colon, prostate gland and fallopian tubes.
Applications:Suitable for use in Western Blot, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human LOST1.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa10-90 of human LOST1 at N-terminal.
