84-5508-65　［取扱停止］LIMK-1/2 30ul　538530

特徴

• There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain.
• LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers.
• Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions.
• LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain.
• LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin.
• LIM domain kinase 1 is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure.
• LIM domain kinase 1 also stimulates axon growth and may play a role in brain development.
• LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.
• Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.