LGMN encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds.
This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems.
Enzyme activation is triggered by acidic pH and appears to be autocatalytic.
Protein expression occurs after monocytes differentiate into dendritic cells.
A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells.
Overexpression of LGMN may be associated with the majority of solid tumor types.
LGMN has a pseudogene on chromosome 13.
Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined.
These two variants encode the same isoform.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human LGMN. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa60-140 from human LGMN.
