84-5507-45　［取扱停止］LATS1/2, phosphorylated (Thr1079/1041) 100ul　538468

特徴

• The protein encoded by LATS1 (large tumor suppressor kinase 1) is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis.
• The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase.
• The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A.
• In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding.
• Biochemical and genetic data suggest a role as a tumor suppressor.
• This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
• Two protein-coding transcripts and one non-protein coding transcript have been found for LATS1.
• Applications:Suitable for use in Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.