84-5507-01　［取扱停止］Laminin-R 100ul　538446

特徴

• Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes.
• They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis.
• Many of the effects of laminin are mediated through interactions with cell surface receptors.
• These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins.
• RPSA encodes a high-affinity, non-integrin family, laminin receptor 1.
• This receptor has been variously called 67kD laminin receptor, 37kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein.
• The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function.
• It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts.
• Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype.
• Multiple copies of RPSA exist, however, most of them are pseudogenes thought to have arisen from retropositional events.
• Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.