Lambda 5 (also called Immunoglobulin lambda-like polypeptide 1 or CD179b antigen) and VpreB comprise the surrogate light chain of the pre-B cell receptor complex.
SL chain is also part of a quality control mechanism that tests a m-chain for its ability to pair with conventional L chains.
It can form Ig-like complexes with the heavy (H) chain, the DHJHC m-protein or the p55 chain.
Production of the surrogate light chain begins at the stage of pro-B cells, continues during the pre-B-cell stage and halts at the immature B cell stage.
Once pre-BCR is expressed, SL chain expression is turned off.
As pre-B II cells proliferate, SL is diluted out, thus limiting pre-BCR formation.
Lambda 5 is critical for B cell development in mammals.
Expression of Lambda 5 is highest in liver, pre- B-lymphocytes and bone marrow, the major source of B cell precursors.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Lambda 5.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human Lambda 5 at C-terminal.
