84-5504-82　［取扱停止］KMT1B 100ul　538323

特徴

• Histone-lysine N-methyltransferase SUV39H2 is a Histone methyltransferase that specifically trimethylates Lys-9 of histone H3 using monomethylated H3 Lys-9 as substrate.
• H3 Lys-9 trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones.
• Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions.
• H3 Lys-9 trimethylation is also required to direct DNA methylation at pericentric repeats.
• SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length.
• May participate in regulation of higher-order chromatin organization during spermatogenesis.
• Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 Lys-9 trimethylation.
• Among its related pathways are Lysine degradation and Chromatin organization.
• Gene Ontology (GO) annotations related to this gene include chromatin binding and transcription regulatory region sequence-specific DNA binding.
• An important paralog of this gene is EHMT1.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.